Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA
- 1 March 1976
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 260 (5548), 221-225
- https://doi.org/10.1038/260221a0
Abstract
DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.Keywords
This publication has 22 references indexed in Scilit:
- Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coliNature, 1975
- Construction and characterization of a chimeric plasmid composed of DNA from Escherichia coli and Drosophila melanogasterBiochemistry, 1975
- Insertion of a rabbit β-globin gene sequence into an E.coli plasmidNucleic Acids Research, 1975
- A system for mapping DNA sequences in the chromosomes of Drosophila melanogasterCell, 1974
- Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNAProceedings of the National Academy of Sciences, 1974
- Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNAProceedings of the National Academy of Sciences of the United States of America, 1974
- The gene numbers gameCell, 1974
- A method for selective cloning of eukaryotic DNA fragments in Escherichia coli by repeated transformationMolecular Genetics and Genomics, 1974
- Replication and Transcription of Eukaryotic DNA in Esherichia coliProceedings of the National Academy of Sciences of the United States of America, 1974
- Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coliProceedings of the National Academy of Sciences, 1972