Viable Molecular Hybrids of Bacteriophage Lambda and Eukaryotic DNA
- 1 November 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (11), 4579-4583
- https://doi.org/10.1073/pnas.71.11.4579
Abstract
A bacteriophage .lambda. strain was constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the .lambda. DNA. These hybrid DNA can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this .lambda. strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages were produced with E. coli DNA and Drosophila melanogaster DNA.This publication has 10 references indexed in Scilit:
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