Virp1 Is a Host Protein with a Major Role in Potato Spindle Tuber Viroid Infection in Nicotiana Plants
- 1 December 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (23), 12872-12880
- https://doi.org/10.1128/jvi.00974-07
Abstract
Viroids are small, circular, single-stranded RNA molecules that, while not coding for any protein, cause several plant diseases. Viroids rely for their infectious cycle on host proteins, most of which are likely to be involved in endogenous RNA-mediated phenomena. Therefore, characterization of host factors interacting with the viroid may contribute to the elucidation of RNA-related pathways of the hosts. Potato spindle tuber viroid (PSTVd) infects several members of the Solanaceae family. In an RNA ligand screening we have previously isolated the tomato protein Virp1 by its ability to specifically interact with PSTVd positive-strand RNA. Virp1 is a bromodomain-containing protein with an atypical RNA binding domain and a nuclear localization signal. Here we investigate the role of Virp1 in the viroid infection cycle by the use of transgenic lines of Nicotiana tabacum and Nicotiana benthamiana that either overexpress the tomato Virp1 RNA or suppress the orthologous Nicotiana genes through RNA silencing. Plants of the Virp1 -suppressed lines were not infected by PSTVd or Citrus exocortis viroid through mechanical inoculation, indicating a major role of Virp1 in viroid infection. On the other hand, overexpression of tomato Virp1 in N. tabacum and N. benthamiana plants did not affect PSTVd KF 440-2 infectivity or symptomatology in these species. Transfection experiments with isolated protoplasts revealed that Virp1 -suppressed cells were unable to sustain viroid replication, suggesting that resistance to viroid infection in Virp1 -suppressed plants is likely the result of cell-autonomous events.Keywords
This publication has 45 references indexed in Scilit:
- A Structured Viroid RNA Serves as a Substrate for Dicer-Like Cleavage To Produce Biologically Active Small RNAs but Is Resistant to RNA-Induced Silencing Complex-Mediated DegradationJournal of Virology, 2007
- Viroid: A Useful Model for Studying the Basic Principles of Infection and RNA BiologyMolecular Plant-Microbe Interactions®, 2007
- Spontaneous short‐range silencing of a GFP transgene in Nicotiana benthamiana is possibly mediated by small quantities of siRNA that do not trigger systemic silencingThe Plant Journal, 2006
- Viroids: petite RNA pathogens with distinguished talentsTrends in Plant Science, 2004
- Direct Role of a Viroid RNA Motif in Mediating Directional RNA Trafficking across a Specific Cellular Boundary[W]Plant Cell, 2004
- Effectiveness of RNA interference in transgenic plantsFEBS Letters, 2004
- Reversible cross-linking combined with immunoprecipitation to study RNA–protein interactions in vivoMethods, 2002
- Potato spindle tuber viroid as Inducer of RNA Silencing in Infected TomatoMolecular Plant-Microbe Interactions®, 2001
- The bromodomain: a chromatin browser?Frontiers in Bioscience-Landmark, 2001
- Cell‐to‐cell movement of potato spindle tuber viroidThe Plant Journal, 1997