AtaA, a New Member of the Trimeric Autotransporter Adhesins from Acinetobacter sp. Tol 5 Mediating High Adhesiveness to Various Abiotic Surfaces
Open Access
- 14 November 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (11), e48830
- https://doi.org/10.1371/journal.pone.0048830
Abstract
Acinetobacter sp. Tol 5 exhibits an autoagglutinating nature and noteworthy adhesiveness to various abiotic surfaces from hydrophobic plastics to hydrophilic glass and stainless steel. Although previous studies have suggested that bacterionanofibers on Tol 5 cells are involved in the adhesive phenotype of Tol 5, the fiber that directly mediates Tol 5 adhesion has remained unknown. Here, we present a new member of trimeric autotransporter adhesins designated AtaA, which we discovered by analyzing a less adhesive mutant of Tol 5, T1, obtained by transposon mutagenesis. AtaA forms thinner and shorter nanofibers than fimbriae on Tol 5 cells. We performed target disruption of ataA by allelic marker exchange, and the resulting ΔataA strain was complemented with ataA on the Escherichia coli-Acinetobacter shuttle vector, which was newly constructed. These results proved that AtaA is essential for Tol 5’s autoagglutinating nature and high adhesiveness to surfaces of various materials. In addition, the adhesiveness to solid surfaces mediated by AtaA is notably higher than that mediated by YadA of Yersinia enterocolitica WA-314. Moreover, and importantly, these characteristics can be conferred to the non-adhesive, non-agglutinating bacterium Acinetobacter sp. ADP1 in trans by transformation with ataA, with expected applications to microbial immobilization.Keywords
This publication has 48 references indexed in Scilit:
- Trimeric Autotransporter Adhesin-Dependent Adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to Matrix Components and Endothelial Cells under Static and Dynamic Flow ConditionsInfection and Immunity, 2011
- Localization of the Domains of the Haemophilus ducreyi Trimeric Autotransporter DsrA Involved in Serum Resistance and Binding to the Extracellular Matrix Proteins Fibronectin and VitronectinInfection and Immunity, 2009
- Architectures and biogenesis of non-flagellar protein appendages in Gram-negative bacteriaThe EMBO Journal, 2008
- The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coilThe EMBO Journal, 2008
- Monolayer Adsorption of a “Bald” Mutant of the Highly Adhesive and Hydrophobic Bacterium Acinetobacter sp. Strain Tol 5 to a Hydrocarbon SurfaceApplied and Environmental Microbiology, 2008
- Domain annotation of trimeric autotransporter adhesins—daTAABioinformatics, 2008
- A Second Aggregatibacter actinomycetemcomitans Autotransporter Adhesin Exhibits Specificity for Buccal Epithelial Cells in Humans and Old World PrimatesInfection and Immunity, 2007
- Adhesion of single bacterial cells in the micronewton rangeProceedings of the National Academy of Sciences, 2006
- Predicting Coiled Coils from Protein SequencesScience, 1991
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983