A Second Aggregatibacter actinomycetemcomitans Autotransporter Adhesin Exhibits Specificity for Buccal Epithelial Cells in Humans and Old World Primates
Open Access
- 1 September 2007
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 75 (9), 4440-4448
- https://doi.org/10.1128/iai.02020-06
Abstract
Previous work showed that the Aggregatibacter actinomycetemcomitans adhesin Aae demonstrated species specificity and tissue tropism to buccal epithelial cells (BECs) derived from humans and Old World primates, but a second, lower-affinity adhesin was noted. This study was designed to determine if Omp100 (also known as ApiA), a surface-expressed A. actinomycetemcomitans adhesin, is that second adhesin and if so to investigate its tissue tropism and species specificity. A targeted mutagenesis protocol was used to construct an isogenic apiA mutant and an aae apiA double mutant with wild-type A. actinomycetemcomitans . In addition, Escherichia coli strain DH5α was used to express apiA to further assess binding parameters. Results indicated that the apiA mutant strain showed significantly less binding to BECs than its parent strain ( P ≤ 0.05). Further, binding mediated by ApiA was specific to BECs from humans and Old World primates, as seen in both wild-type A. actinomycetemcomitans and E. coli expressing ApiA ( P ≤ 0.05). Pretreatment of wild-type A. actinomycetemcomitans cells with anti-ApiA antiserum reduced binding in a dose-dependent manner. The aae apiA double mutant completely abrogated A. actinomycetemcomitans binding to both human and Old World primate BECs. Taken together, these studies indicate that ApiA and Aae, in concert, modulate binding of A. actinomycetemcomitans to human BECs. Since the BEC is a prominent reservoir for A. actinomycetemcomitans , identification of this second adhesin could lead to important therapeutic strategies.Keywords
This publication has 23 references indexed in Scilit:
- Genetic and Functional Analyses of the Actinobacillus actinomycetemcomitans AfeABCD Siderophore-Independent Iron Acquisition SystemInfection and Immunity, 2005
- The Actinobacillus actinomycetemcomitans Autotransporter Adhesin Aae Exhibits Specificity for Buccal Epithelial Cells from Humans and Old World PrimatesInfection and Immunity, 2005
- Escherichia colibind to urinary bladder epithelium through nonspecific sialic acid mediated adherenceFEMS Immunology & Medical Microbiology, 2003
- Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous β-Hexosaminidase ActivityJournal of Bacteriology, 2003
- Timing the Origin of New World MonkeysMolecular Biology and Evolution, 2003
- Lactoferrin Iron Levels Affect Attachment ofActinobacillus actinomycetemcomitansto Buccal Epithelial CellsThe Journal of Periodontology, 2002
- Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in Buccal Epithelial Cells Collected from Human SubjectsInfection and Immunity, 2001
- Simultaneous isolation of Actinobacillus actinomycetemcomitans from subgingival and extracrevicular locations of the mouthJournal of Clinical Periodontology, 1995
- Sub‐gingival microflora in Macaca mulatta species of rhesus monkeyJournal of Periodontal Research, 1993
- Comparative studies on the biology of Actinobacillus actinomycetemcomitans leukotoxin in primatesOral Microbiology and Immunology, 1987