The Asexual Pathogen Aspergillus fumigatus Expresses Functional Determinants of Aspergillus nidulans Sexual Development
- 1 October 2008
- journal article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 7 (10), 1724-1732
- https://doi.org/10.1128/ec.00157-08
Abstract
The major fungal pathogen of humans, Aspergillus fumigatus , lacks a defined sexual cycle, although the presence of genes encoding putative mating type idiomorphs and regulators of Aspergillus sexual development heightens the potential for cryptic sexuality in this deuteromycete. To test the functionality of these genetic determinants, we transferred the alpha box-encoding mat1-1 idiomorph from an A. fumigatus isolate to the homothallic fertile species Aspergillus nidulans . Abundant formation of fruiting bodies (cleistothecia) containing viable ascospores establishes functionality of this mating type gene product in the transgenic strain. Using a similar approach, we also established that the conserved transcriptional regulator from A. fumigatus , the nsdD gene product, can act as a functional, positively acting factor for A. nidulans cleistothecium development; moreover, high-level expression of NsdD in the endogenous host A. fumigatus profoundly alters hyphal development by triggering the formation of coiled hyphae. Our findings demonstrate that the presumably asexual pathogen A. fumigatus encodes functional regulators of mating and sexual development, thereby potentiating the case for cryptic sexuality in this fungal pathogen.Keywords
This publication has 42 references indexed in Scilit:
- Mating Type Protein Mat1-2 from Asexual Aspergillus fumigatus Drives Sexual Reproduction in Fertile Aspergillus nidulansEukaryotic Cell, 2008
- Genomic Islands in the Pathogenic Filamentous Fungus Aspergillus fumigatusPLoS Genetics, 2008
- Mating Type and the Genetic Basis of Self-Fertility in the Model Fungus Aspergillus nidulansCurrent Biology, 2007
- Aspergillus genomes: secret sex and the secrets of sexTrends in Genetics, 2006
- Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzaeNature, 2005
- CRYPTIC SPECIATION IN THE COSMOPOLITAN AND CLONAL HUMAN PATHOGENIC FUNGUS ASPERGILLUS FUMIGATUSEvolution, 2005
- Sexual reproduction between partners of the same mating type in Cryptococcus neoformansNature, 2005
- Mating type gene homologues in Aspergillus fumigatusMicrobiology, 2003
- Sexual Development in Ascomycetes Fruit Body Formation of Aspergillus nidulansPublished by Taylor & Francis Ltd ,2002
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985