Mating Type Protein Mat1-2 from Asexual Aspergillus fumigatus Drives Sexual Reproduction in Fertile Aspergillus nidulans
Open Access
- 1 June 2008
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 7 (6), 1029-1040
- https://doi.org/10.1128/ec.00380-07
Abstract
The lack of an experimentally amenable sexual genetic system in Aspergillus fumigatus is a major limitation in the study of the organism's pathogenesis. A recent comparative genome analysis revealed evidence for potential sexuality in A. fumigatus . Homologs of mating type genes as well as other genes of the “sexual machinery” have been identified in anamorphic A. fumigatus . The mat1 - 2 gene encodes a homolog of MatA, an HMG box mating transcriptional factor (Mat HMG ) that regulates sexual development in fertile Aspergillus nidulans . In this study, the functionalities of A. fumigatus mat1 - 2 and the Mat1-2 protein were determined by interspecies gene exchange between sterile A. fumigatus and fertile A. nidulans . Ectopically integrated A. fumigatus mat1 - 2 (driven by its own promoter) was not functional in a sterile A. nidulans Δ matA strain, and no sexual development was observed. In contrast, the A. fumigatus mat1 - 2 open reading frame driven by the A. nidulans matA promoter and integrated by homologous gene replacement at the matA locus was functional and conferred full fertility. This is the first report showing that cross species mating type gene exchange between closely related Ascomycetes did not function in sexual development. This is also the first report demonstrating that a Mat HMG protein from an asexual species is fully functional, with viable ascospore differentiation, in a fertile homothallic species. The expression of mat1 - 2 was assessed in A. fumigatus and A. nidulans . Our data suggest that mat1 - 2 may not be properly regulated to allow sexuality in A. fumigatus . This study provides new insights about A. fumigatus asexuality and also suggests the possibility for the development of an experimentally amenable sexual cycle.Keywords
This publication has 53 references indexed in Scilit:
- Mating Type and the Genetic Basis of Self-Fertility in the Model Fungus Aspergillus nidulansCurrent Biology, 2007
- DNA Sequence Characterization and Molecular Evolution of MAT1 and MAT2 Mating-Type Loci of the Self-Compatible Ascomycete Mold Neosartorya fischeriEukaryotic Cell, 2007
- Pathogenesis ofAspergillus fumigatusand the Kinetics of Galactomannan in an In Vitro Model of Early Invasive Pulmonary Aspergillosis: Implications for Antifungal TherapyThe Journal of Infectious Diseases, 2007
- The Predicted G-Protein-Coupled Receptor GPR-1 Is Required for Female Sexual Development inthe Multicellular Fungus Neurospora crassaEukaryotic Cell, 2006
- Low Genetic Variation and No Detectable Population Structure in Aspergillus fumigatus Compared to Closely Related Neosartorya SpeciesEukaryotic Cell, 2006
- Microarray and real-time PCR analyses reveal mating type-dependent gene expression in a homothallic fungusMolecular Genetics and Genomics, 2006
- Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzaeNature, 2005
- Fungal Genomics: Forensic Evidence of Sexual ActivityCurrent Biology, 2005
- Mating type gene homologues in Aspergillus fumigatusMicrobiology, 2003
- Structure and function of mating type genes inCochliobolusspp. and asexual fungiCanadian Journal of Botany, 1995