In vivo inhibition of neutrophil function in the rabbit using monoclonal antibody to CD18.
- 15 December 1987
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 139 (12), 4174-4177
- https://doi.org/10.4049/jimmunol.139.12.4174
Abstract
The CD11b/CD18 membrane antigen complex has been shown to be essential for normal neutrophil function in vitro, and patients lacking this antigen exhibit severe neutrophil dysfunction. Murine monoclonal antibody 60.3 (Ab 60.3), directed toward CD18, is a potent inhibitor of human neutrophil function in vitro. To determine whether Ab 60.3 might similarly inhibit neutrophil function in vivo, we measured the effect of antibody administration on neutrophil localization to polyvinyl sponges placed s.c., in rabbits. Studies in 25 animals showed that infusion of Ab 60.3 at the time of sponge insertion resulted in dose-dependent inhibition of in vivo neutrophil migration with almost complete paralysis of neutrophil migration at higher antibody doses. These studies confirm the functional relevance of CD18, demonstrate that neutrophil function can be profoundly inhibited in vivo by Ab 60.3, and suggest that substances such as AB 60.3 may be clinically useful as potent anti-inflammatory agents.This publication has 22 references indexed in Scilit:
- ABSENCE OF MONOCLONAL-ANTIBODY-DEFINED PROTEIN COMPLEX IN BOY WITH ABNORMAL LEUCOCYTE FUNCTIONThe Lancet, 1984
- Deficiency of a surface membrane glycoprotein (Mo1) in man.JCI Insight, 1984
- Definition of a common leukocyte cell-surface antigen (Lp95-150) associated with diverse cell-mediated immune functions.The Journal of Immunology, 1983
- A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.The Journal of Experimental Medicine, 1983
- Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte-granulocyte membrane glycoprotein (Mol).JCI Insight, 1983
- Severe recurrent bacterial infections associated with defective adherence and chemotaxis in two patients with neutrophils deficient in a cell-associated glycoproteinThe Journal of Pediatrics, 1982
- Deficiency of a Granulocyte-Membrane Glycoprotein (gp150) in a Boy with Recurrent Bacterial InfectionsNew England Journal of Medicine, 1982
- Neutrophil-endothelial cell interactions on endothelial monolayers grown on micropore filters.JCI Insight, 1981
- An Inherited Abnormality of Neutrophil AdhesionNew England Journal of Medicine, 1980
- Neutrophil preservation: the effect of short-term storage on in vivo kinetics.JCI Insight, 1977