A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
Open Access
- 1 December 1983
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 158 (6), 1785-1803
- https://doi.org/10.1084/jem.158.6.1785
Abstract
The human lymphocyte function-associated antigen-1 (LFA-1), the complement receptor-associated OKM1 molecule, and a previously undescribed molecule termed p150,95, have been found to be structurally and antigenically related. Each antigen contains an alpha- and beta-subunit noncovalently associated in an alpha 1 beta 1-structure as shown by cross-linking experiments. LFA-1, OKM1, and p150,95 alpha-subunit designations and their molecular weights are alpha L = 177,000 Mr, alpha M = 165,000 Mr, and alpha X = 150,000 Mr, respectively. The beta-subunits are all = 95,000 Mr. Some MAb precipitated only LFA-1, others only OKM1, and another precipitates all three antigens. The specificity of these MAb for particular subunits was examined after subunit dissociation by high pH. MAb specific for LFA-1 or OKM1 bind to the alpha L- or alpha M-subunits, respectively, while the cross-reactive MAb binds to the beta-subunits. Coprecipitation experiments with intact alpha 1 beta 1-complexes showed anti-alpha and anti-beta MAb can precipitate the same molecules. In two-dimensional (2D) isoelectric focusing-SDS-PAGE, the alpha subunits of the three antigens are distinct, while the beta-subunits are identical. Biosynthesis experiments showed alpha L, alpha M, and alpha X are synthesized from distinct precursors, as is beta. The three antigens differ in expression on lymphocytes, granulocytes, and monocytes. During maturation of the monoblast-like U937 line, alpha M and alpha X are upregulated and alpha L is downregulated. Some MAb to the alpha subunit of OKM1 inhibited the complement receptor type three. LFA-1, OKM1, and p150,95 constitute a novel family of functionally important human leukocyte antigens that share a common beta-subunit.Keywords
This publication has 37 references indexed in Scilit:
- A human lymphocyte‐associated antigen involved in cell‐mediated lympholysisEuropean Journal of Immunology, 1983
- Severe recurrent bacterial infections associated with defective adherence and chemotaxis in two patients with neutrophils deficient in a cell-associated glycoproteinThe Journal of Pediatrics, 1982
- Deficiency of a Granulocyte-Membrane Glycoprotein (gp150) in a Boy with Recurrent Bacterial InfectionsNew England Journal of Medicine, 1982
- Response of normal subjects to mitogens: I. Influence of adherent cellsClinical Immunology and Immunopathology, 1981
- Interaction of target cell-bound C3bi and C3d with human lymphocyte receptors. Enhancement of antibody-mediated cellular cytotoxicity.The Journal of Experimental Medicine, 1981
- Delineation of an effector population responsible for natural killing and antibody-dependent cellular cytotoxicity in manClinical Immunology and Immunopathology, 1981
- An Inherited Abnormality of Neutrophil AdhesionNew England Journal of Medicine, 1980
- Mac‐1: a macrophage differentiation antigen identified by monoclonal antibodyEuropean Journal of Immunology, 1979
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- Maturation of the head of bacteriophage T4: I. DNA packaging eventsJournal of Molecular Biology, 1973