An improved mRFP1 adds red to bimolecular fluorescence complementation
Open Access
- 21 July 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 3 (8), 597-600
- https://doi.org/10.1038/nmeth901
Abstract
Protein-protein interactions are fundamental to virtually every aspect of cellular functions. Blue, green and yellow bimolecular fluorescence complementation (BiFC) systems based on GFP and its variants allow the investigation of protein-protein interactions in vivo. We have developed the first red BiFC system based on an improved monomeric red fluorescent protein (mRFP1-Q66T), expanding the range of possible applications for BiFC.This publication has 13 references indexed in Scilit:
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