Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
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- 3 May 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 296 (5569), 913-916
- https://doi.org/10.1126/science.1068539
Abstract
Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating this segregation of proteins in the membrane are poorly understood. We suppressed the tendency of Aequorea fluorescent proteins to dimerize and targeted these variants to the plasma membrane using several different types of lipid anchors. Fluorescence resonance energy transfer measurements in living cells revealed that acyl but not prenyl modifications promote clustering in lipid rafts. Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane.Keywords
This publication has 21 references indexed in Scilit:
- Cholesterol Depletion Disrupts Caveolae and Insulin Receptor Signaling for Metabolic Control via Insulin Receptor Substrate-1, but Not for Mitogen-activated Protein Kinase ControlOnline Journal of Public Health Informatics, 2001
- Differential Targeting of β-Adrenergic Receptor Subtypes and Adenylyl Cyclase to Cardiomyocyte CaveolaeOnline Journal of Public Health Informatics, 2000
- MODERN APPLICATIONS OF ANALYTICAL ULTRACENTRIFUGATIONAnnual Review of Biophysics and Biophysical Chemistry, 1999
- Role of Lipid Modifications in Targeting Proteins to Detergent-resistant Membrane RaftsOnline Journal of Public Health Informatics, 1999
- Distribution of a Glycosylphosphatidylinositol-anchored Protein at the Apical Surface of MDCK Cells Examined at a Resolution of <100 Å Using Imaging Fluorescence Resonance Energy TransferThe Journal of cell biology, 1998
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- The molecular structure of green fluorescent proteinNature Biotechnology, 1996
- Co-purification and Direct Interaction of Ras with Caveolin, an Integral Membrane Protein of Caveolae MicrodomainsOnline Journal of Public Health Informatics, 1996
- A detergent-free method for purifying caveolae membrane from tissue culture cells.Proceedings of the National Academy of Sciences of the United States of America, 1995
- Protein Lipidation in Cell SignalingScience, 1995