Quantitative Detection of Human Adenoviruses in Wastewater and Combined Sewer Overflows Influencing a Michigan River
- 1 February 2010
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 76 (3), 715-723
- https://doi.org/10.1128/aem.01316-09
Abstract
Enteric viruses are important pathogens found in contaminated surface waters and have previously been detected in waters of the Great Lakes. Human adenoviruses were monitored because of their high prevalence and persistence in aquatic environments. In this study, we quantified adenoviruses in wastewater, surface water, and combined sewer overflows (CSOs) by real-time PCR. Between August 2005 and August 2006, adenovirus concentrations in raw sewage, primary-treated effluent, secondary-treated effluent, and chlorinated effluent from a wastewater treatment plant in Michigan were examined. CSO samples ( n = 6) were collected from a CSO retention basin in Grand Rapids, MI. Adenoviruses were detected in 100% of wastewater and CSO discharge samples. Average adenovirus DNA concentrations in sewage and CSOs were 1.15 × 10 6 viruses/liter and 5.35 × 10 5 viruses/liter, respectively. Adenovirus removal was 10 (99%) at the wastewater treatment plant. Adenovirus type 41 (60% of clones), type 12 (29%), type 40 (3%), type 2 (3%), and type 3 (3%) were isolated from raw sewage and primary effluents ( n = 28). Six of 20 surface water samples from recreational parks at the lower Grand River showed virus concentrations above the real-time PCR detection limit (average, 7.8 × 10 3 viruses/liter). This research demonstrates that wastewater effluents and wastewater-impacted surface waters in the lower Grand River in Michigan contain high levels of viruses and may not be suitable for full-body recreational activities. High concentrations of adenovirus in these waters may be due to inefficient removal during wastewater treatment and to the high persistence of these viruses in the environment.Keywords
This publication has 59 references indexed in Scilit:
- Molecular Detection and Characterization of Gastroenteritis Viruses Occurring Naturally in the Stream Waters of Manaus, Central Amazônia, BrazilApplied and Environmental Microbiology, 2008
- Evaluation of Removal of Noroviruses during Wastewater Treatment, Using Real-Time Reverse Transcription-PCR: Different Behaviors of Genogroups I and IIApplied and Environmental Microbiology, 2007
- Occurrence of Human Adenoviruses at Two Recreational Beaches of the Great LakesApplied and Environmental Microbiology, 2007
- Genotype Prevalence and Risk Factors for Severe Clinical Adenovirus Infection, United States 2004-2006Clinical Infectious Diseases, 2007
- Massive Microbiological Groundwater Contamination Associated with a Waterborne Outbreak in Lake Erie, South Bass Island, OhioEnvironmental Health Perspectives, 2007
- Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater MatricesApplied and Environmental Microbiology, 2006
- Real-Time Reverse Transcription-PCR for Detection of Rotavirus and Adenovirus as Causative Agents of Acute Viral Gastroenteritis in ChildrenJournal of Clinical Microbiology, 2006
- Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon geneArchiv für die gesamte Virusforschung, 2006
- Existence of multiple outbreaks of viral gastroenteritis among infants in a day care center in JapanArchiv für die gesamte Virusforschung, 2005
- Assessment of an Enterovirus Sewage SurveillanceSystem by Comparison of Clinical Isolates with Sewage Isolatesfrom Milwaukee, Wisconsin, Collected August 1994 toDecember2002Applied and Environmental Microbiology, 2003