Radiographic videodensitometry for quantitative monitoring of experimental bone healing
- 1 October 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in The British Journal of Radiology
- Vol. 62 (742), 883-889
- https://doi.org/10.1259/0007-1285-62-742-883
Abstract
A method for non-invasive assessment of experimental bone healing in animals is described. Optimized radiographs were analysed by digital processing using a computer-based image analysis system. Values of the total mass were expressed as te equivalent mass of a simultaneously exposed aluminium roentgen-density reference. In order to assess the potential of the method, measurements were initially performed on phantoms and bone specimens. The systematic error was negligible. An excellent correlation (r = 0.999) was found between the densitometric weight (.mu.) and the corresponding dry weight (m) of the analysed bone samples and a calibration factor .mu./m = 1.38 was established. The total random error was low, the coefficient of variation (CV) amounting to > 5% or, if duplicate analyses were performed, > 3%. The accuracy was not impaired when radiography was carried out with addition of water to mimic overlying soft tissue in the model. The method was applied to the assessment of callus mass longitudinally in vivo in the healing of a bone defect in the rabbit radius, yielding reproducible values (CV < 5%) and a quantitative analysis of the healing sequence.This publication has 20 references indexed in Scilit:
- Recurrent bone regeneration in titanium implants. Experimental model for determining the healing capacity of bone using quantitative microradiographyBiomaterials, 1988
- Coralline Hydroxyapatite Bone-Graft Substitutes in a Canine Diaphyseal Defect ModelInvestigative Radiology, 1987
- Radiographic image analysis in the study of bone morphologyClinical Physics and Physiological Measurement, 1987
- Percutaneous Bone Marrow Grafting of Fractures and Bony DefectsPublished by Ovid Technologies (Wolters Kluwer Health) ,1986
- Pulverized Bone Matrix as an Injectable Bone Graft in Rabbit Radius DefectsClinical Orthopaedics and Related Research, 1986
- Determination of callus quantity in 4‐ week‐old fractures of the rat tibiaJournal of Orthopaedic Research, 1985
- Osteogenetic Activity in Composite Grafts of Demineralized Compact Bone and MarrowPublished by Ovid Technologies (Wolters Kluwer Health) ,1983
- Bone Formation of Transplanted Autologous Bone Matrix in Rabbit Evaluated by Technetium Radionuclide Bone ImagingScandinavian Journal of Plastic and Reconstructive Surgery, 1982
- Problems in the Measurement of Bone DensityThe British Journal of Radiology, 1951
- A Method for the Quantitative Roentgenological Determination of the Amount of Calcium Salts in Bone Tissue:Its Use in the Study of Calcium Reduction in the Phalanges of the Fingers in Chronic PolyarthritisActa Radiologica, 1949