Prokaryotic Communities in Pit Mud from Different-Aged Cellars Used for the Production of Chinese Strong-Flavored Liquor
Open Access
- 1 April 2014
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 80 (7), 2254-2260
- https://doi.org/10.1128/aem.04070-13
Abstract
Chinese strong-flavored liquor (CSFL) accounts for more than 70% of all Chinese liquor production. Microbes in pit mud play key roles in the fermentation cellar for the CSFL production. However, microbial diversity, community structure, and cellar-age-related changes in pit mud are poorly understood. Here, we investigated the prokaryotic community structure and diversity in pit-mud samples with different cellar ages (1, 10, 25, and 50 years) using the pyrosequencing technique. Results indicated that prokaryotic diversity increased with cellar age until the age reached 25 years and that prokaryotic community structure changed significantly between three cellar ages (1, 10, and 25 years). Significant correlations between prokaryotic communities and environmental variables (pH, NH4 +, lactic acid, butyric acid, and caproic acid) were observed. Overall, our study results suggested that the long-term brewing operation shapes unique prokaryotic community structure and diversity as well as pit-mud chemistry. We have proposed a three-phase model to characterize the changes of pit-mud prokaryotic communities. (i) Phase I is an initial domestication period. Pit mud is characterized by abundant Lactobacillus and high lactic acid and low pH levels. (ii) Phase II is a transition period. While Lactobacillus abundance decreases dramatically, that of Bacteroidetes and methanogens increases. (iii) Phase III is a relative mature period. The prokaryotic community shows the highest diversity and capability to produce more caproic acid as a precursor for synthesis of ethyl caproate, the main flavor component in CSFL. This research provides scientific evidence to support the practical experience that old fermentation cellars produce high-quality liquor.Keywords
This publication has 28 references indexed in Scilit:
- Pyrosequencing Analysis of Bacterial Diversity in 14 Wastewater Treatment Systems in ChinaApplied and Environmental Microbiology, 2012
- Analysis of 16S rRNA Amplicon Sequencing Options on the Roche/454 Next-Generation Titanium Sequencing PlatformPLOS ONE, 2011
- Disentangling the importance of ecological niches from stochastic processes across scalesPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2011
- UCHIME improves sensitivity and speed of chimera detectionBioinformatics, 2011
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental ScaleApplied and Environmental Microbiology, 2009
- Environmental and anthropogenic controls over bacterial communities in wetland soilsProceedings of the National Academy of Sciences of the United States of America, 2008
- Impact of pH on Lactate Formation and Utilization by Human Fecal Microbial CommunitiesApplied and Environmental Microbiology, 2007
- Degradation of Cyanophycin by Sedimentibacter hongkongensis Strain KI and Citrobacter amalonaticus Strain G Isolated from an Anaerobic Bacterial ConsortiumApplied and Environmental Microbiology, 2005
- Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoirInternational Journal of Systematic and Evolutionary Microbiology, 2005