Pyrosequencing Analysis of Bacterial Diversity in 14 Wastewater Treatment Systems in China
Top Cited Papers
Open Access
- 1 October 2012
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (19), 7042-7047
- https://doi.org/10.1128/aem.01617-12
Abstract
To determine if there is a core microbial community in the microbial populations of different wastewater treatment plants (WWTPs) and to investigate the effects of wastewater characteristics, operational parameters, and geographic locations on microbial communities, activated sludge samples were collected from 14 wastewater treatment systems located in 4 cities in China. High-throughput pyrosequencing was used to examine the 16S rRNA genes of bacteria in the wastewater treatment systems. Our results showed that there were 60 genera of bacterial populations commonly shared by all 14 samples, including Ferruginibacter , Prosthecobacter , Zoogloea , Subdivision 3 genera incertae sedis , Gp4, Gp6, etc., indicating that there is a core microbial community in the microbial populations of WWTPs at different geographic locations. The canonical correspondence analysis (CCA) results showed that the bacterial community variance correlated most strongly with water temperature, conductivity, pH, and dissolved oxygen (DO) content. Variance partitioning analyses suggested that wastewater characteristics had the greatest contribution to the bacterial community variance, explaining 25.7% of the variance of bacterial communities independently, followed by operational parameters (23.9%) and geographic location (14.7%). Results of this study provided insights into the bacterial community structure and diversity in geographically distributed WWTPs and discerned the relationships between bacterial community and environmental variables in WWTPs.Keywords
This publication has 33 references indexed in Scilit:
- 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plantsThe ISME Journal, 2011
- Patterns of fungal diversity and composition along a salinity gradientThe ISME Journal, 2010
- Functional gene diversity of soil microbial communities from five oil-contaminated fields in ChinaThe ISME Journal, 2010
- Combined niche and neutral effects in a microbial wastewater treatment communityProceedings of the National Academy of Sciences of the United States of America, 2010
- Vertical stratification of microbial communities in the Red Sea revealed by 16S rDNA pyrosequencingThe ISME Journal, 2010
- Discovery of commonly existing anode biofilm microbes in two different wastewater treatment MFCs using FLX Titanium pyrosequencingApplied Microbiology and Biotechnology, 2010
- Diversity and population structure of sewage‐derived microorganisms in wastewater treatment plant influentEnvironmental Microbiology, 2010
- A bacterial metapopulation adapts locally to phage predation despite global dispersalGenome Research, 2007
- Global patterns in bacterial diversityProceedings of the National Academy of Sciences of the United States of America, 2007
- Microbial diversity in the deep sea and the underexplored “rare biosphere”Proceedings of the National Academy of Sciences of the United States of America, 2006