Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology
- 21 December 2006
- journal article
- review article
- Published by Wiley in The Journal of Physiology
- Vol. 578 (1), 55-67
- https://doi.org/10.1113/jphysiol.2006.120212
Abstract
This article reviews genetically encoded Ca2+ indicators (GECIs), with a focus on the use of these novel molecules in the context of understanding complex cell signalling in mammals, in vivo. The review focuses on the advantages and limitations of specific GECI design strategies and the results of experiments in which these molecules have been expressed in transgenic mice, concentrating particularly on recent experiments from our laboratory in which physiological signalling could be monitored in vivo. Finally, newer strategies for effective genetic specification of GECIs are briefly reviewed.This publication has 71 references indexed in Scilit:
- Local subplasma membrane Ca 2+ signals detected by a tethered Ca 2+ sensorProceedings of the National Academy of Sciences of the United States of America, 2006
- Direct in vivo monitoring of sarcoplasmic reticulum Ca2+ and cytosolic cAMP dynamics in mouse skeletal muscleThe Journal of cell biology, 2006
- Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinNature Biotechnology, 2004
- Expanded dynamic range of fluorescent indicators for Ca 2+ by circularly permuted yellow fluorescent proteinsProceedings of the National Academy of Sciences of the United States of America, 2004
- Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET ControlPLoS Biology, 2004
- Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live CellsScience, 2002
- Preassociation of Calmodulin with Voltage-Gated Ca2+ Channels Revealed by FRET in Single Living CellsNeuron, 2001
- Circularly permuted green fluorescent proteins engineered to sense Ca 2+Proceedings of the National Academy of Sciences of the United States of America, 2001
- Red Fluorescent Protein from Discosoma as a Fusion Tag and a Partner for Fluorescence Resonance Energy TransferBiochemistry, 2001
- Kinetics of calcium binding to fluo-3 determined by stopped-flow fluorescenceBiochemical and Biophysical Research Communications, 1989