Regulation of the Expression of Plant Resistance Gene SNC1 by a Protein with a Conserved BAT2 Domain
Open Access
- 3 May 2010
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 153 (3), 1425-1434
- https://doi.org/10.1104/pp.110.156240
Abstract
Plant Resistance (R) genes encode immune receptors that recognize pathogens and activate defense responses. Because of fitness costs associated with maintaining R protein-mediated resistance, expression levels of R genes have to be tightly regulated. However, mechanisms on how R-gene expression is regulated are poorly understood. Here we show that MODIFIER OF snc1, 1 (MOS1) regulates the expression of SUPPRESSOR OF npr1-1, CONSTITUTIVE1 (SNC1), which encodes a Toll/interleukin receptor-nucleotide binding site-leucine-rich repeat type of R protein in Arabidopsis (Arabidopsis thaliana). In the mos1 loss-of-function mutant plants, snc1 expression is repressed and constitutive resistance responses mediated by snc1 are lost. The repression of snc1 expression in mos1 is released by knocking out DECREASE IN DNA METHYLATION1. In mos1 mutants, DNA methylation in a region upstream of SNC1 is altered. Furthermore, expression of snc1 transgenes using the native promoter does not require MOS1, indicating that regulation of SNC1 expression by MOS1 is at the chromatin level. Map-based cloning of MOS1 revealed that it encodes a novel protein with a HLA-B ASSOCIATED TRANSCRIPT2 (BAT2) domain that is conserved in plants and animals. Our study on MOS1 suggests that BAT2 domain-containing proteins may function in regulation of gene expression at chromatin level.Keywords
This publication has 29 references indexed in Scilit:
- Gene Duplication and Hypermutation of the Pathogen Resistance Gene SNC1 in the Arabidopsis bal VariantGenetics, 2009
- A Novel Role for Protein Farnesylation in Plant Innate ImmunityPlant Physiology, 2008
- The Expression Pattern of a Rice Disease Resistance Gene Xa3/Xa26 Is Differentially Regulated by the Genetic Backgrounds and Developmental Stages That Influence Its FunctionGenetics, 2007
- The ubiquitin pathway is required for innate immunity in ArabidopsisThe Plant Journal, 2007
- MOS2, a Protein Containing G-Patch and KOW Motifs, Is Essential for Innate Immunity in Arabidopsis thalianaCurrent Biology, 2005
- Activation Tagging in ArabidopsisPlant Physiology, 2000
- Identification and Cloning of a Negative Regulator of Systemic Acquired Resistance, SNI1, through a Screen for Suppressors of npr1-1Cell, 1999
- Maintenance of genomic methylation requires a SWI2/SNF2-like proteinNature Genetics, 1999
- The DNA methylation locusDDM1 is required for maintenance of gene silencing in ArabidopsisGenes & Development, 1998
- PLANT DISEASE RESISTANCE GENESAnnual Review of Plant Physiology and Plant Molecular Biology, 1997