Activation Tagging in Arabidopsis
Top Cited Papers
- 1 April 2000
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 122 (4), 1003-1014
- https://doi.org/10.1104/pp.122.4.1003
Abstract
Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant mutants with various phenotypes have been isolated. Analysis of a subset of mutants has shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to 3.6 kb. In at least one case, the CaMV 35S enhancers led primarily to an enhancement of the endogenous expression pattern rather than to constitutive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the complete CaMV 35S promoter. This has important implications for the spectrum of genes that will be discovered by this method.Keywords
This publication has 52 references indexed in Scilit:
- A Pair of Related Genes with Antagonistic Roles in Mediating Flowering SignalsScience, 1999
- The Arabidopsis Dwarf Mutant shi Exhibits Reduced Gibberellin Responses Conferred by Overexpression of a New Putative Zinc Finger ProteinTHE PLANT CELL ONLINE, 1999
- ReviewBiological Chemistry, 1997
- A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2.THE PLANT CELL ONLINE, 1996
- A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in DrosophilaCell, 1993
- Phenotypic and Genetic Analysis of det2, a New Mutant That Affects Light-Regulated Seedling Development in Arabidopsis.THE PLANT CELL ONLINE, 1991
- Ectopic expression from the Deformed gene triggers a dominant defect in Drosophila adult head developmentDevelopmental Biology, 1990
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Developmental studies of lethality associated with the Antennapedia gene complex in Drosophila melanogasterDevelopmental Biology, 1981
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962