Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy
- 1 January 2010
- journal article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 15 (2), 027008
- https://doi.org/10.1117/1.3374049
Abstract
The goal of this study is to determine the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for delineation of brain tumor from surrounding normal tissue in order to assist the neurosurgeon in near-complete tumor excision. A time-domain TR-LIFS prototype apparatus (gated photomultiplier detection, fast digitizer) was used for recording tissue autofluorescence in normal cortex (NC), normal white matter (NWM), and various grades of gliomas intraoperatively. Tissue fluorescence was induced with a pulsed nitrogen laser (337 nm, 700 ps), and the intensity decay profiles were recorded in the 360- to 550-nm spectral range (10-nm interval). Histopathological analysis (hematoxylin & eosin) of the biopsy samples taken from the site of TR-LIFS measurements was used for validation of spectroscopic results. Preliminary results on 17 patients demonstrate that normal cortex (N=16) and normal white matter (N=3) show two peaks of fluorescence emission at 390 nm (lifetime=1.8±0.3 ns) and 460 nm (lifetime=0.8±0.1 ns). The 390-nm emission peak is absent in low-grade glioma (N=5; lifetime=1.1 ns) and reduced in high-grade glioma (N=9; lifetime=1.7±0.4 ns). The emission characteristics at 460 nm in all tissues correlated with the nicotinamide adenine dinucleotide fluorescence (peak: 440 to 460 nm; lifetime: 0.8 to 1.0 ns). These findings demonstrate the potential of using TR-LIFS as a tool for enhanced delineation of brain tumors during surgery. In addition, this study evaluates similarities and differences between TR-LIFS signatures of brain tumors obtained in vivo and those previously reported in ex vivo brain tumor specimens.Keywords
This publication has 37 references indexed in Scilit:
- The 2007 WHO Classification of Tumours of the Central Nervous SystemActa Neuropathologica, 2007
- Distinction of brain tissue, low grade and high grade glioma with time-resolved fluorescence spectroscopyFrontiers in Bioscience-Landmark, 2006
- Diagnosis of meningioma by time-resolved fluorescence spectroscopyJournal of Biomedical Optics, 2005
- Flavoprotein Autofluorescence Imaging of Neuronal Activation in the Cerebellar Cortex In VivoJournal of Neurophysiology, 2004
- Glutamic acid decarboxylase immunoreactive large neuron types in the granular layer of the human cerebellar cortexBrain Structure and Function, 2004
- Fast model-free deconvolution of fluorescence decay for analysis of biological systemsJournal of Biomedical Optics, 2004
- Fluorescence Lifetime Spectroscopy of Glioblastoma Multiforme¶Photochemistry and Photobiology, 2004
- Discrimination of Human Coronary Artery Atherosclerotic Lipid-Rich Lesions by Time-Resolved Laser-Induced Fluorescence SpectroscopyArteriosclerosis, Thrombosis, and Vascular Biology, 2001
- Absence of Pyridoxine-5‘-Phosphate Oxidase (PNPO) Activity in Neoplastic Cells: Isolation, Characterization, and Expression of PNPO cDNABiochemistry, 1998
- Laser-induced fluorescence: experimental intraoperative delineation of tumor resection marginsJournal of Neurosurgery, 1992