Distinction of brain tissue, low grade and high grade glioma with time-resolved fluorescence spectroscopy
- 1 January 2006
- journal article
- Published by IMR Press in Frontiers in Bioscience-Landmark
- Vol. 11 (1), 1255-63
- https://doi.org/10.2741/1878
Abstract
Neuropathology frozen section diagnoses are difficult in part because of the small tissue samples and the paucity of adjunctive rapid intraoperative stains. This study aims to explore the use of time-resolved laser-induced fluorescence spectroscopy as a rapid adjunctive tool for the diagnosis of glioma specimens and for distinction of glioma from normal tissues intraoperatively. Ten low grade gliomas, 15 high grade gliomas without necrosis, 6 high grade gliomas with necrosis and/or radiation effect, and 14 histologically uninvolved "normal" brain specimens are spectroscopicaly analyzed and contrasted. Tissue autofluorescence was induced with a pulsed Nitrogen laser (337 nm, 1.2 ns) and the transient intensity decay profiles were recorded in the 370-500 nm spectral range with a fast digitized (0.2 ns time resolution). Spectral intensities and time-dependent parameters derived from the time-resolved spectra of each site were used for tissue characterization. A linear discriminant analysis diagnostic algorithm was used for tissue classification. Both low and high grade gliomas can be distinguished from histologically uninvolved cerebral cortex and white matter with high accuracy (above 90%). In addition, the presence or absence of treatment effect and/or necrosis can be identified in high grade gliomas. Taking advantage of tissue autofluorescence, this technique facilitates a direct and rapid investigation of surgically obtained tissue.Keywords
This publication has 16 references indexed in Scilit:
- Effects of fiber-optic probe design and probe-to-target distance on diffuse reflectance measurements of turbid media: an experimental and computational study at 337 nmApplied Optics, 2004
- Fast model-free deconvolution of fluorescence decay for analysis of biological systemsJournal of Biomedical Optics, 2004
- Fluorescence Lifetime Spectroscopy of Glioblastoma Multiforme¶Photochemistry and Photobiology, 2004
- Diagnostic potential of autofluorescence for an assisted intraoperative delineation of glioblastoma resection margins.2003
- Discrimination of Human Coronary Artery Atherosclerotic Lipid-Rich Lesions by Time-Resolved Laser-Induced Fluorescence SpectroscopyArteriosclerosis, Thrombosis, and Vascular Biology, 2001
- QUANTITATIVE OPTICAL SPECTROSCOPY FOR TISSUE DIAGNOSISAnnual Review of Physical Chemistry, 1996
- Identification of nonlinear biological systems using laguerre expansions of kernelsAnnals of Biomedical Engineering, 1993
- Laser-induced fluorescence: experimental intraoperative delineation of tumor resection marginsJournal of Neurosurgery, 1992
- FLUORESCENCE IMAGING AND POINT MEASUREMENTS OF TISSUE: APPLICATIONS TO THE DEMARCATION OF MALIGNANT TUMORS AND ATHEROSCLEROTIC LESIONS FROM NORMAL TISSUEPhotochemistry and Photobiology, 1991
- Autofluorescence of viable cultured mammalian cells.Journal of Histochemistry & Cytochemistry, 1979