Requirement for the SLP-76 Adaptor GADS in T Cell Development
- 9 March 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 291 (5510), 1987-1991
- https://doi.org/10.1126/science.1057176
Abstract
GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS – CD4 – CD8 – thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS – thymocytes failed to respond to CD3 cross-linking in vivo and were impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS – thymocytes. These observations indicate that GADS is a critical adaptor for CD3 signaling.Keywords
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