Lability of Host-Cell DNA in Growing Cell Cultures Due to Mycoplasma
- 3 September 1965
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 149 (3688), 1098-1099
- https://doi.org/10.1126/science.149.3688.1098
Abstract
In HeLa-cell cultures chronically infected with Mycoplasma, (PPLO) host-cell DNA is unstable as detected by incorporation of H3-or C1 i-thymidine into DNA and subsequent release into the medium as acidsoluble radioactivity. This characteristic can be transmitted to PPLO-free cultures of strain L cells by inoculation with preparations of PPLO from the HeLa cells, although chronically infected cultures of L cells continue to multiply. In addition, virus preparations also may carry PPLO contamination through numerous passages.Keywords
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