ARGININE METABOLISM IN PLEUROPNEUMONIA-LIKE ORGANISMS ISOLATED FROM MAMMALIAN CELL CULTURE
- 1 August 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 86 (2), 195-+
- https://doi.org/10.1128/jb.86.2.195-206.1963
Abstract
Arginine degradation is a significant metabolic process for pleuropneumonia-like organisms (PPLO; Mycoplasma) isolated from cell culture. The conversion of arginine to ornithine in PPLO-contaminated cell culture was rapid, and occurred by the arginine dihydrolase pathway involving arginine deiminase, ornithine transcarbamylase and carbamyl phosphokinase. In the absence of PPLO contamination, arginine conversion to ornithine was minimal and took place by an arginase activity present in the cell culture but not in the PPLO. All five PPLO strains isolated from cell culture accomplished the conversion of arginine to ornithine and contained the requisite enzyme of the arginine dihydrolase system, whereas PPLO-free cell cultures did not. Supplementation of PPLO culture broth with arginine increased the extent of PPLO growth. When the arginine content of the culture limited growth, arginine was completely converted to ornithine. When growth was limited in the presence of excess arginine, citrulline was the major breakdown product. It is suggested that the conversion of arginine to ornithine constitutes a significant and possible major source of adenosine triphosphate for this class of organisms.Keywords
This publication has 44 references indexed in Scilit:
- OXIDATIVE METABOLISM OF PLEUROPNEUMONIA-LIKE ORGANISMS*Annals of the New York Academy of Sciences, 2006
- Repression of enzymes of arginin biosynthesis in mammalian tissue cultureBiochimica et Biophysica Acta, 1962
- The Growth of Micro-organisms in Relation to their Energy SupplyMicrobiology, 1960
- AMINO ACID METABOLISM OF PPLO*Annals of the New York Academy of Sciences, 1960
- PRODUCTS OF GLUCOSE METABOLISM BY PLEUROPNEUMONIALIKE ORGANISMS*Annals of the New York Academy of Sciences, 1960
- Amino Acid Metabolism in Mammalian Cell CulturesScience, 1959
- Contamination of Stock Lines of Human Carcinoma Cells by Pleuropneumonia-like OrganismsNature, 1957
- Contamination of Human Cell Cultures by Pleuropneumonialike OrganismsScience, 1956
- Sur l'argininedésiminisae et sur la formation enzymatique de citrulline par les levuresBiochimica et Biophysica Acta, 1952
- Über den Abbau des Arginins zu Citrullin durch Bacillus pyocyaneus.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1933