Ex vivo cytotoxicity of a new calcium silicate–based canal filling material

Abstract

Zhang W, Li Z, Peng B. Ex vivo cytotoxicity of a new calcium silicate–based canal filling material. International Endodontic Journal, 43, 769–774, 2010. Abstract Aim To evaluate the biocompatibility of iRoot SP root canal filling material to L929 mouse fibroblasts. Methodology Specimens of iRoot SP, AH Plus and ProRoot mineral trioxide aggregate (MTA) were prepared for direct contact using the Millipore filter diffusion test, as well as extracts in MTT assay. Mouse fibroblasts (L929) were used as toxicity targets. Differences in cytotoxicity between fresh and set specimens and between root canal material extracts were determined by t-test. A P-value P < 0.05). In addition, iRoot SP was significantly more toxic than MTA (P < 0.05). In the MTT assay with set specimens, extracts of iRoot SP and MTA were noncytotoxic, whilst extracts of AH Plus were rated as slightly cytotoxic. AH Plus was significantly more toxic than iRoot SP and MTA (P < 0.05). Conclusion AH Plus root canal sealer was significantly more toxic to L-929 cells than MTA and iRoot SP. iRoot SP had intermediate toxicity.