Alishewanella agri sp. nov., isolated from landfill soil

Abstract

Strain BL06^Twas isolated from landfill soil in Pohang, Korea. Strain BL06^Tis Gram-negative, aerobic, non-motile and rod-shaped. For growth, the NaCl range is 0–6 % (w/v), the temperature range is 10–44 °C and the pH range is 5.5–12.0. Based on the 16S rRNA gene and gyrase B (gyrB) gene sequences, phylogenetic analysis showed that strain BL06^Tis associated with the genusAlishewanellaand related closely to the type strains ofAlishewanellaspecies (98.8 % 16S rRNA gene sequence similarity toAlishewanella aestuarii, 98.7 % toAlishewanella fetalisand 98.5 % toAlishewanella jeotgali). Physiological and biochemical tests verified that strain BL06^Tis genotypically and phenotypically different from previously described species in the genusAlishewanella. DNA–DNA hybridization experiments showed that relatedness between the genomic DNA of strain BL06^Tand type strains of otherAlishewanellaspecies is Tand related strains. Therefore, strain BL06^T(=KCTC 22400^T=JCM 15597^T) is proposed to represent a novel species in the genusAlishewanella, namedAlishewanella agrisp. nov.