Alishewanella jeotgali sp. nov., isolated from traditional fermented food, and emended description of the genus Alishewanella
- 20 July 2009
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 59 (9), 2313-2316
- https://doi.org/10.1099/ijs.0.007260-0
Abstract
A novel Gram-negative and facultative anaerobic strain, designated MS1T, was isolated from gajami sikhae, a traditional fermented food in Korea made from flatfish. Strain MS1T was motile, rod-shaped and oxidase- and catalase-positive, and required 1–2 % (w/v) NaCl for growth. Growth occurred at temperatures ranging from 4 to 40 °C and the pH range for optimal growth was pH 6.5–9.0. Strain MS1T was capable of reducing trimethylamine oxide, nitrate and thiosulfate. Phylogenetic analysis placed strain MS1T within the genus Alishewanella. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MS1T was related closely to Alishewanella aestuarii B11T (98.67 % similarity) and Alishewanella fetalis CCUG 30811T (98.04 % similarity). However, DNA–DNA reassociation experiments between strain MS1T and reference strains showed relatedness values <70 % (42.6 and 14.8 % with A. aestuarii B11T and A. fetalis CCUG 30811T, respectively). Genotypic, physiological and biochemical analyses allowed the differentiation of strain MS1T from type strains of species belonging to the genus Alishewanella. Therefore, we propose that strain MS1T (=KCTC 22429T =JCM 15561T) is assigned to a novel species, Alishewanella jeotgali sp. nov.Keywords
This publication has 11 references indexed in Scilit:
- Alishewanella aestuarii sp. nov., isolated from tidal flat sediment, and emended description of the genus AlishewanellaInternational Journal of Systematic and Evolutionary Microbiology, 2009
- MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0Molecular Biology and Evolution, 2007
- Review and re-analysis of domain-specific 16S primersJournal of Microbiological Methods, 2003
- A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperatureEnvironmental Microbiology, 2002
- Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus.International Journal of Systematic and Evolutionary Microbiology, 2000
- The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Research, 1997
- Improved immobilization of DNA to microwell plates for DNA-DNA hybridizationNucleic Acids Research, 1996
- Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in BacteriologyInternational Journal of Systematic and Evolutionary Microbiology, 1994
- Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial StrainsInternational Journal of Systematic and Evolutionary Microbiology, 1989
- Evolutionary trees from DNA sequences: A maximum likelihood approachJournal of Molecular Evolution, 1981