Cloning and sequence analysis of cDNA for rat corticotropin‐releasing factor precursor
- 21 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (1), 63-66
- https://doi.org/10.1016/0014-5793(85)80994-7
Abstract
DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.Keywords
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