Predicting the clonogenic survival of A549 cells after modulated x-ray irradiation using the linear quadratic model
- 16 December 2008
- journal article
- Published by IOP Publishing in Physics in Medicine & Biology
- Vol. 54 (2), 187-206
- https://doi.org/10.1088/0031-9155/54/2/002
Abstract
In this study we present two prediction methods, mean dose and summed dose, for predicting the number of A549 cells that will survive after modulated x-ray irradiation. The prediction methods incorporate the dose profile from the modulated x-ray fluence map applied across the cell sample and the linear quadratic (LQ) model. We investigated the clonogenic survival of A549 cells when irradiated using two different modulated x-ray fluence maps. Differences between the measured and predicted surviving fraction were observed for modulated x-ray irradiation. When the x-ray fluence map produced a steep dose gradient across the sample, fewer cells survived in the unirradiated region than expected. When the x-ray fluence map produced a less steep dose gradient across the sample, more cells survived in the unirradiated region than expected. Regardless of the steepness of the dose gradient, more cells survived in the irradiated region than expected for the reference dose range of 1-10 Gy. The change in the cell survival for the unirradiated regions of the two different dose gradients may be an important factor to consider when predicting the number of cells that will survive at the edge of modulated x-ray fields. This investigation provides an improved method of predicting cell survival for modulated x-ray radiation treatment. It highlights the limitations of the LQ model, particularly in its ability to describe the biological response of cells irradiated under these conditions.Keywords
This publication has 29 references indexed in Scilit:
- Effect of dose rate on the radiation-induced bystander responsePhysics in Medicine & Biology, 2007
- Clonogenic assay of cells in vitroNature Protocols, 2006
- A preliminary investigation of cell growth after irradiation using a modulated x-ray intensity patternPhysics in Medicine & Biology, 2006
- SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture SystemMedical Physics, 2005
- Delivery time comparison for intensity-modulated radiation therapy with/without flattening filter: a planning studyPhysics in Medicine & Biology, 2004
- A proliferation-dependent bystander effect in primary porcine and human urothelial explants in response to targeted irradiationBritish Journal of Cancer, 2003
- Comparative evaluation of Kodak EDR2 and XV2 films for verification of intensity modulated radiation therapyPhysics in Medicine & Biology, 2002
- Cellular communication and bystander effects: a critical review for modelling low-dose radiation actionMutation Research - Reviews in Mutation Research, 2002
- Some characteristics of tumour control probability for heterogeneous tumoursPhysics in Medicine & Biology, 1996
- A molecular theory of cell survivalPhysics in Medicine & Biology, 1973