Inactivation of α1 ‐antiproteinase by hydroxyl radicals The effect of uric acid
- 13 February 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 244 (1), 76-80
- https://doi.org/10.1016/0014-5793(89)81166-4
Abstract
The elastase-inhibitory activity of α,-antiproteinase is inactivated by hydroxyl radicals (.OH) generated by pulse radiolysis or by reaction of iron ions with H2O2 in the presence of superoxide or ascorbate. Uric acid did not protect α1-antiproteinase against inactivation by .OH in pulse radiolysis experiments or in the superoxide/iron/H2O2 system, whereas it did in systems containing ascorbic acid. We propose that radicals formed by attack of .OH on uric acid are themselves able to inactivate α1 1-antiproteinase, but that these uric acid radicals can be ‘repaired’ by ascorbic acid.Keywords
This publication has 34 references indexed in Scilit:
- The iron-binding and hydroxyl radical scavenging action of anti-inflammatory drugsXenobiotica, 1988
- Biologically significant scavenging of the myeloperoxidase‐derived oxidant hypochlorous acid by ascorbic acidFEBS Letters, 1987
- Myeloperoxidase oxidation of sulfur‐centered and benzoic acid hydroxyl radical scavengersFEBS Letters, 1985
- Formation of hydroxyl radicals from NADH and NADPH in the presence of copper saltsJournal of Inorganic Biochemistry, 1985
- Oxygen-Derived Free Radicals in Postischemic Tissue InjuryNew England Journal of Medicine, 1985
- The Determination of Dehydroascorbic Acid and Ascorbic acid in the Serum and Synovial Fluid of Patients with Rheumatoid Arthritis (RA)Free Radical Research Communications, 1985
- Urate and ascorbate: their possible roles as antioxidants in determining longevity of mammalian speciesArchives of Gerontology and Geriatrics, 1984
- Inhibition of free radical‐induced DNA damage by uric acidFEBS Letters, 1984
- Uric acid substantially enhances the free radical‐induced inactivation of alcohol dehydrogenaseFEBS Letters, 1984
- Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis.Proceedings of the National Academy of Sciences, 1981