Isolation and HPLC Quantitative Analysis of Flavonoid Glycosides from Brazilian Beverages (Maytenus ilicifoliaandM.aquifolium)

Abstract

Aqueous infusions of Brazilian Maytenus leaves are used as beverages, foodstuffs, and phytomedicines. Previously, we isolated two new flavonoid tetrasaccharides from the infusion of Maytenus aquifolium leaves that showed antiulcer activity. In this investigation a new flavonoid tetrasaccharide, kaempferol-3-O-α-l-rhamnopyranosyl (1→6)-O-[α-l-arabinopyranosyl (1→3)-O-α-l-rhamnopyranosyl (1→2)]-O-β-d-galactopyranoside (3), was isolated, together with kaempferol tri- and disaccharides and quercetin trisaccharides from the aqueous infusion of Maytenus ilicifolia leaves. All structures were elucidated by ES-MS and NMR spectroscopic methods. The quantitative analysis of the flavonoid glycosides from Maytenusilicifolia and M.aquifolium has been performed by HPLC. Keywords: Maytenus ilicifolia; M. aquifolium Martius; Celastraceae; espinheira santa; infusion; beverage; flavonoids glycosides; 1D and 2D NMR; quantitative determination