In vitro and in vivo effects of potassium and magnesium on storage up to 7 days of apheresis platelet concentrates in platelet additive solution
- 23 November 2007
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 94 (2), 96-102
- https://doi.org/10.1111/j.1423-0410.2007.01002.x
Abstract
Prolonged storage of platelets up to 7 days provides improved availability, logistical management and decreased wastage. Beside methods of bacterial detection, addition of magnesium and potassium to the platelet storage solution (SSP+) may further improve the quality of platelets with extended storage. Apheresis platelets from 10 donors were divided and stored in two different platelet additive solutions (PAS) (Intersol and SSP+) for a paired comparison. A variety of in vitro platelet function and metabolic assays were performed both on day 1 and after 7 days of storage. For in vivo study, platelets were labelled with either (111)Indium or (51)Chromium after 7 days of storage and were injected into the corresponding donor. Serial blood samples were drawn for recovery and survival measurements. In vitro parameters for SSP+ showed significantly reduced glycolysis (lower glucose consumption and decreased production of lactate), a higher hypotonic shock response (HSR) and the extent of shape change reactivity and a lower degree of platelet activation by means of RANTES (regulated on activation, normal, T cell-expressed, and secreted), CD62p and CD63 expression. Platelet recovery on day 7 was higher for Intersol as compared to SSP+, 65 +/- 11 vs. 53 +/- 13% (P = 0.023), and survival showed no difference 4.2 +/- 1.9 vs. 3.6 +/- 1.4 days. In vitro characteristics of platelets stored in PAS with addition of potassium and magnesium indicated higher quality, but this could not be verified by the in vivo parameters by means of recovery and survival.Keywords
This publication has 47 references indexed in Scilit:
- Storage of buffy‐coat‐derived platelets in additive solutions at 4 °C and 22 °C: flow cytometry analysis of platelet glycoprotein expressionVox Sanguinis, 2007
- Paired in vitro and in vivo comparison of apheresis platelet concentrates stored in platelet additive solution for 1 versus 7 daysTransfusion, 2006
- Further evaluation of a new standard of efficacy for stored plateletsTransfusion, 2005
- Platelet storage solution effects on the accuracy of laboratory tests for platelet function: a multi‐laboratory studyVox Sanguinis, 2004
- The Role of the Plasma from Platelet Concentrates in Transfusion ReactionsNew England Journal of Medicine, 1994
- Validation of use of the Nageotte hemocytometer to count low levels of white cells in white cell-reduced platelet componentsTransfusion, 1994
- Activation during preparation and storage of platelet concentratesTransfusion, 1992
- Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusionTransfusion, 1991
- Evaluation of platelets prepared by apheresis and stored for 5 days. In vitro and in vivo studiesTransfusion, 1989
- Poststorage platelet viability in thrombocytopenic recipients is reliably measured by radiochromium‐labeled platelet recovery and survival measurements in normal volunteersTransfusion, 1986