Storage of buffy‐coat‐derived platelets in additive solutions at 4 °C and 22 °C: flow cytometry analysis of platelet glycoprotein expression
- 7 April 2007
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 93 (1), 27-36
- https://doi.org/10.1111/j.1423-0410.2007.00912.x
Abstract
The aim of our in vitro study is to compare the effects on platelet membrane glycoproteins that play an important role in the main functions of platelets, when platelets are stored for a period of 21 days at 4 degrees C or 22 degrees C. Platelet concentrates (PC) were prepared from pooled buffy-coats (BC) for paired studies (total eight pools from 80 BCs) by using the OrbiSac system. We divided each pool into two PCs and stored them at 4 degrees C or 22 degrees C. The activation marker CD62 remained almost unchanged during storage in all units. The expression of CD63 was higher in PCs stored at 22 degrees C than in those stored at 4 degrees C. No significant difference in CD41 expression was detected over time. The expression of CD42b declined during storage and even more in PCs stored at 4 degrees C until day 21 [day 14: mean flourscence intensity: 32.5 +/- 13.1 vs. 46.5 +/- 19.1], but the percentage of platelets expressing CD42b remained high in platelets stored at 4 degrees C, but gradually decreased at 22 degrees C (day 14: 95.0 +/- 1.5 vs. 59.0 +/- 9.9). Storage at 4 degrees C reduced the rate of glycolysis and maintained the pH better after day 10 than in PCs stored at 22 degrees C (day 14: 7.009 +/- 0.067 vs. 7.233 +/- 0.125). The concentration of regulated upon activation of normal T-cells expressed and secreted was higher in PCs stored at 22 degrees C than at 4 degrees C (day 7: 414.7 +/- 32.3 vs. 49.6 +/- 19.0). No response to extent of shape change and no swirling were detected at 4 degrees C. Platelets stored at 4 degrees C retain their in vitro characteristics better than those stored at 22 degrees C, except for parameters that reflect changes in shape. Storage at 4 degrees C is not associated with an increased expression of glycoprotein (GpIb, GpIIb/IIIa) and platelet activation markers (CD62p and CD63) as compared with storage at 22 degrees C.Keywords
This publication has 36 references indexed in Scilit:
- Assessment of the correlation of platelet morphology with in vivo recovery and survivalTransfusion, 2005
- Automated preparation of platelet concentrates from pooled buffy coats: in vitro studies and experiences with the OrbiSac systemTransfusion, 2005
- Platelet storage solution effects on the accuracy of laboratory tests for platelet function: a multi‐laboratory studyVox Sanguinis, 2004
- The Clearance Mechanism of Chilled Blood PlateletsCell, 2003
- Mechanisms of Cold-induced Platelet Actin AssemblyOnline Journal of Public Health Informatics, 2001
- Validation of use of the Nageotte hemocytometer to count low levels of white cells in white cell-reduced platelet componentsTransfusion, 1994
- Activation in stored platelet concentrates: correlation between membrane expression of P‐selectin, glycoprotein IIb/IIIa, and beta‐ thromboglobulin releaseTransfusion, 1993
- Activation during preparation and storage of platelet concentratesTransfusion, 1992
- The cellular and molecular basis of the platelet storage lesion: a symposium summaryTransfusion, 1992
- Studies of Platelet Concentrates Stored at 22 C and 4 CTransfusion, 1973