The Dual-Specificity Phosphatase CDC14B Bundles and Stabilizes Microtubules
Open Access
- 1 June 2005
- journal article
- Published by Informa UK Limited in Molecular and Cellular Biology
- Vol. 25 (11), 4541-4551
- https://doi.org/10.1128/mcb.25.11.4541-4551.2005
Abstract
The Cdc14 dual-specificity phosphatases regulate key events in the eukaryotic cell cycle. However, little is known about the function of mammalian CDC14B family members. Here, we demonstrate that subcellular localization of CDC14B protein is cell cycle regulated. CDC14B can bind, bundle, and stabilize microtubules in vitro independently of its catalytic activity. Basic amino acid residues within the nucleolar targeting domain are important for both retaining CDC14B in the nucleolus and preventing microtubule bundling. Overexpression of CDC14B resulted in the formation of cytoplasmic CDC14B and microtubule bundles in interphase cells. These microtubule bundles were resistant to microtubule depolymerization reagents and enriched in acetylated α-tubulin. Expression of cytoplasmic forms of CDC14B impaired microtubule nucleation from the microtubule organization center. CDC14B is thus a novel microtubule-bundling and -stabilizing protein, whose regulated subcellular localization may help modulate spindle and microtubule dynamics in mitosis.Keywords
This publication has 74 references indexed in Scilit:
- Stabilization of microtubule dynamics at anaphase onset promotes chromosome segregationNature, 2005
- Visualization of a highly organized intranuclear network of filaments in living mammalian cellsCell Motility, 2004
- Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2The Journal of cell biology, 2004
- The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryoThe Journal of cell biology, 2002
- The Projection Domain of MAP4 Suppresses the Microtubule-bundling Activity of the Microtubule-binding DomainJournal of Molecular Biology, 2002
- Centrosome-Dependent Exit of Cytokinesis in Animal CellsScience, 2001
- On the Regulation and Function of Human Polo-like Kinase 1 (PLK1): Effects of Overexpression on Cell Cycle ProgressionBiochemical and Biophysical Research Communications, 1997
- Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells [published erratum appears in J Cell Biol 1996 Dec;135(6 Pt 1):1679]The Journal of cell biology, 1996
- Differential Taxol-dependent arrest of transformed and nontransformed cells in the G1 phase of the cell cycle, and specific-related mortality of transformed cells.The Journal of cell biology, 1996
- Tubulin dynamics in cultured mammalian cells.The Journal of cell biology, 1984