Midzone microtubule bundles are continuously required for cytokinesis in cultured epithelial cells [published erratum appears in J Cell Biol 1996 Dec;135(6 Pt 1):1679]
Open Access
- 1 November 1996
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 135 (4), 981-989
- https://doi.org/10.1083/jcb.135.4.981
Abstract
The current model of cytokinesis proposes that spindle poles and associated microtubules determine the cleavage plane, and, once the signal has been delivered to the cortex, the entire mitotic apparatus can be removed without affecting cell division. While supported by compelling data from Echinoderm embryos, recent observations suggest that the model may not be universally applicable. In this study, we have examined the relationship(s) among microtubules, chromosomes, and cleavage activity in living normal rat kidney (NRK) cells with multipolar mitotic figures. We found that cleavage activity correlated with the distribution of midzone microtubule bundles and Telophase Disc 60 protein (TD60) rather than the position of spindle poles. In addition, reduction of midzone microtubules near the cortex, by either nocodazole treatment or spontaneous reorganization in tripolar cells, caused inhibition or regression of furrowing. These results demonstrate that continuous interaction between midzone microtubule bundles and the cortex is required for successful cleavage in tissue culture cells.Keywords
This publication has 49 references indexed in Scilit:
- [36] Preparation of tubulin from brainMethods in enzymology, 1982
- Organization of Actin Meshworks in Cultured Cells: The Leading EdgeCold Spring Harbor Symposia on Quantitative Biology, 1982
- Anaphase progression and furrow establishment in nocodazole-arrested PtK1 cellsChromosoma, 1981
- The origin of cleavage forces in dividing eggs: A mechanism in two stepsExperimental Cell Research, 1981
- Organization of actin in the leading edge of cultured cells: influence of osmium tetroxide and dehydration on the ultrastructure of actin meshworks.The Journal of cell biology, 1981
- Cell cleavageExperimental Cell Research, 1979
- Microdissection studies on the dividing neuroblast of the grasshopper, with special reference to the mechanism of unequal cytokinesisExperimental Cell Research, 1977
- Improved techniques for the induction of mammalian cell hybridization by polyethylene glycolSomatic Cell and Molecular Genetics, 1976
- Establishment of cleavage furrows by the mitotic spindleJournal of Experimental Zoology, 1974
- The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B.1970