Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)
Open Access
- 1 January 1989
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (7), 2503-2516
- https://doi.org/10.1093/nar/17.7.2503
Abstract
We have improved the “polymerase chain reaction” (PCR) to permit rapid analysis of any known mutation in genomic DNA. We demonstrate a system, ARMS (Amplification Refractory Mutation System), that allows genotyping solely by inspection of reaction mixtures after agarose gel electrophoresis. The system is simple, reliable and non-isotopic. It will clearly distinguish heterozygotes at a locus from homozygotes for either allele. The system requires neither restriction enzyme digestion, allelespecific oligonucleotides as conventionally applied, nor the sequence analysis of PCR products. The basis of the invention is that unexpectedly, oligonucleotides with a mismatched 3′-residue will not function as primers in the PCR under appropriate conditions. We have analysed DNA from patients with α1-antitrypsin (AAT) deficiency, from carriers of the disease and from normal individuals. Our findings are in complete agreement with allele assignments derived by direct sequencing of PCR products.This publication has 25 references indexed in Scilit:
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