Rat Marrow Stromal Cells are More Sensitive to Plating Density and Expand More Rapidly from Single‐Cell‐Derived Colonies than Human Marrow Stromal Cells
- 1 May 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 19 (3), 219-225
- https://doi.org/10.1634/stemcells.19-3-219
Abstract
Human marrow stromal cell (hMSCs) were recently shown to expand rapidly in culture when plated at a low density of approximately 3 cells/cm(2). Low-density plating promoted proliferation of small recycling stem (RS) cells that appeared to be the most multipotent cells in the cultures. Here we demonstrated that MSCs from rat bone marrow (rMSCs) are even more sensitive to low-density plating than hMSCS: When plated at approximately 2 cells/cm(2), the cells expanded over 4,000-fold in 12 days, over twice the maximal rate observed with hMSCS: Analysis by fluorescence-activated cell sorter demonstrated that rMSCs had the same heterogeneity seen with hMSCs in that the cultures contained both small rapidly RS cells and much larger mature cells (mMSCs). The rat mMSCs differed from human mMSCs in that they regenerated RS cells in culture. Also, after low-density plating, colonies of rMSCs expanded into confluent cultures, whereas colonies of hMSCs did not.Keywords
This publication has 30 references indexed in Scilit:
- Adult Bone Marrow Stromal Cells Differentiate into Neural Cells in VitroExperimental Neurology, 2000
- Adult rat and human bone marrow stromal cells differentiate into neuronsJournal of Neuroscience Research, 2000
- Multipotential Marrow Stromal Cells Transduced to ProduceL-DOPA: Engraftment in a Rat Model of Parkinson DiseaseHuman Gene Therapy, 1999
- Dystrophin expression in the mdx mouse restored by stem cell transplantationNature, 1999
- In Vitro Expansion of a Multipotent Population of Human Neural Progenitor CellsExperimental Neurology, 1999
- Episomal Vectors Rapidly and Stably Produce High-Titer Recombinant RetrovirusHuman Gene Therapy, 1996
- The Tetrahymena chaperonin subunit CCTη gene is coexpressed with CCTγ gene during cilia biogenesis and cell sexual reproductionFEBS Letters, 1996
- Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.The Journal of Experimental Medicine, 1996
- Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5‐azacytidineMuscle & Nerve, 1995
- Characterization of Bone Marrow Stromal Cells in Suspension and Monolayer CulturesBritish Journal of Haematology, 1980