The Tetrahymena chaperonin subunit CCTη gene is coexpressed with CCTγ gene during cilia biogenesis and cell sexual reproduction
- 1 April 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 383 (3), 277-283
- https://doi.org/10.1016/0014-5793(96)00240-2
Abstract
We report here the cloning and the characterization of the T. pyriformis CCTη gene (TpCCTη) and also a partial sequence of the corresponding T. thermophila gene (TtCCTη). The TpCCTη gene encodes a protein sharing a 60.3% identity with the mouse CCTη. We have studied the expression of these genes in Tetrahymena exponentially growing cells, cells regenerating their cilia for different periods and during different stages of the cell sexual reproduction. These genes have similar patterns of expression to those of the previously identified TpCCTγ gene. Indeed, the Tetrahymena CCTη and CCTγ genes are upregulated at 60–120 min of cilia recovery, and in conjugation when vegetative growth was resumed and cell division took place. Our results seem to indicate that both CCT subunits play an important role in the biogenesis of the newly synthesized cilia of Tetrahymena and during its cell division.This publication has 37 references indexed in Scilit:
- The Chaperonin Containing t-complex polypeptide 1 (TCP-1). Multisubunit Machinery Assisting in Protein Folding and Assembly in the Eukaryotic CytosolJBIC Journal of Biological Inorganic Chemistry, 1995
- The Thermosome of Thermoplasma acidophilum and Its Relationship to the Eukaryotic Chaperonin TRiCJBIC Journal of Biological Inorganic Chemistry, 1995
- The Thermosome of Thermoplasma acidophilum and Its Relationship to the Eukaryotic Chaperonin TRiCJBIC Journal of Biological Inorganic Chemistry, 1995
- Identification of six Tcp-1-related genes encoding divergent subunits of the TCP-1-containing chaperoninCurrent Biology, 1994
- Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1Philosophical Transactions Of The Royal Society B-Biological Sciences, 1993
- TCP1 complex is a molecular chaperone in tubulin biogenesisNature, 1992
- Sequence of one α- and two β-tubulin genes of Tetrahymena pyriformis: Structural and functional relationships with other eukaryotic tubulin genesJournal of Molecular Biology, 1988
- Cilia regeneration in starved tetrahymena: an inducible system for studying gene expression and organelle biogenesisCell, 1979
- Rapidly Labelled RNA in Tetrahymena pyriformisJBIC Journal of Biological Inorganic Chemistry, 1975
- Host participation in bacteriophage lambda head assemblyJournal of Molecular Biology, 1973