Chiral imaging of collagen by second-harmonic generation circular dichroism
Open Access
- 20 May 2013
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 4 (6), 909-916
- https://doi.org/10.1364/boe.4.000909
Abstract
We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules.Keywords
This publication has 42 references indexed in Scilit:
- Nonlinear Optical Macroscopic Assessment of 3-D Corneal Collagen Organization and Axial BiomechanicsInvestigative Ophthalmology & Visual Science, 2011
- Collagenous Extracellular Matrix of Cartilage Submitted to Mechanical Forces Studied by Second Harmonic Generation MicroscopyPhotochemistry and Photobiology, 2010
- Chirality in Nonlinear OpticsAnnual Review of Physical Chemistry, 2009
- Three‐dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopyMicroscopy Research and Technique, 2006
- Interpreting Second-Harmonic Generation Images of Collagen I FibrilsBiophysical Journal, 2005
- Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organismsNature Biotechnology, 2003
- Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generationNature Medicine, 2003
- Folding of peptide models of collagen and misfolding in diseaseCurrent Opinion in Structural Biology, 1999
- Collagen-Based Structures Containing the Peptoid ResidueN-Isobutylglycine (Nleu): Synthesis and Biophysical Studies of Gly-Nleu-Pro Sequences by Circular Dichroism and Optical RotationBiochemistry, 1997
- Second harmonic generation in collagenThe Journal of Chemical Physics, 1979