Collagenous Extracellular Matrix of Cartilage Submitted to Mechanical Forces Studied by Second Harmonic Generation Microscopy
- 1 March 2010
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 86 (2), 302-310
- https://doi.org/10.1111/j.1751-1097.2009.00648.x
Abstract
International audienceOsteoarthritis is a degenerative pathology leading to degradation of the extracellular matrix (ECM). Similar effects can be visualized when applying mechanical or biochemical constraints on cartilaginous tissue. Here, we characterized modification of the ECM appearing under mechanical compression and/or biochemical action (hypoxia environment, nitric oxide and collagenase action). In recent decades, multiphoton microscopy has proved its interest for observing living, thick and opaque biological tissues. Thus, the main components of the cartilaginous ECM can be observed without fluorescent labeling. In particular, the collagen network emits strong second harmonic generation (SHG) signal which could be collected at half of the excitation wavelength. Combining autofluorescence and SHG signal detection enables to obtain complementary structural information. Here, we proved that multiphoton microscopy represents an appropriate tool for ex vitro cartilage imaging. First, we showed that SHG signal specifically comes from collagen (collagenase digestion). Further, we verified that the use of an appropriate band-pass filter enables to reject the autofluorescence from the ECM. Once this specificity was shown, we followed modification of the cartilage ECM submitted to mechanical or biochemical constraints (compression, enzymatic digestion). By performing textural analysis of SHG images (Haralick's method), we showed the restructuration of the collagen network according to constraintsKeywords
This publication has 32 references indexed in Scilit:
- Zonal changes in the three-dimensional morphology of the chondron under compression: The relationship among cellular, pericellular, and extracellular deformation in articular cartilageJournal of Biomechanics, 2007
- Biochemical and functional modulation of the cartilage collagen network by IGF1, TGFβ2 and FGF2Osteoarthritis and Cartilage, 2006
- Microstructural Modeling of Collagen Network Mechanics and Interactions with the Proteoglycan Gel in Articular CartilageBiomechanics and Modeling in Mechanobiology, 2006
- A degeneration-based hypothesis for interpreting fibrillar changes in the osteoarthritic cartilage matrixJournal of Anatomy, 2001
- Effect of load on articular cartilage matrix and the development of guinea-pig osteoarthritisOsteoarthritis and Cartilage, 2001
- Degradation of type II collagen, but not proteoglycan, correlates with matrix metalloproteinase activity in cartilage explant culturesArthritis & Rheumatism, 1997
- Damage to type II collagen in aging and osteoarthritis starts at the articular surface, originates around chondrocytes, and extends into the cartilage with progressive degeneration.JCI Insight, 1995
- Proteoglycan-fibrillar collagen interactionsBiochemical Journal, 1988
- An enzymatically induced structural transformation in articular cartilage. its significance with respect to matrix breakdownArthritis & Rheumatism, 1988
- Structural consequences of traumatizing articular cartilage.Annals Of The Rheumatic Diseases, 1986