Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification
- 14 July 2014
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 86 (15), 7843-7848
- https://doi.org/10.1021/ac502276w
Abstract
Sensitive detection of cancer cells plays a critically important role in the early detection of cancer and cancer metastasis. However, because circulating tumor cells are extremely rare in peripheral blood, the detection of cancer cells with high analytical sensitivity and specificity remains challenging. Here, we have demonstrated a simple, sensitive and specific detection of cancer cells with the detection sensitivity of four cancer cells, which is lower than the cutoff value with respect to correlation with survival outcomes as well as predictive of metastatic disease in clinical diagnostics. We re-engineered the hybridization chain reaction (HCR) to multibranched HCR (mHCR) that can produce long products with multiple biotins for signal amplification and multiple branched arms for multivalent binding. The capturing gold surface is modified with DNA tetrahedral probes, which provide superior hybridization conditions for the multivalent binding. The synergetic effect of mHCR amplification and multivalent binding lead to the high sensitivity of our detection platform.Keywords
Funding Information
- National Natural Science Foundation of China (21175155)
- Chinese Academy of Sciences
- Ministry of Science and Technology of the People's Republic of China (2012CB932600)
- Science and Technology Commission of Shanghai Municipality (13PJ1410700)
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