Aptamer-Conjugated Nanoparticles for Cancer Cell Detection
- 10 January 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 83 (3), 727-734
- https://doi.org/10.1021/ac102263v
Abstract
Aptamer-conjugated nanoparticles (ACNPs) have been used for a variety of applications, particularly dual nanoparticles for magnetic extraction and fluorescent labeling. In this type of assay, silica-coated magnetic and fluorophore-doped silica nanoparticles are conjugated to highly selective aptamers to detect and extract targeted cells in a variety of matrixes. However, considerable improvements are required in order to increase the selectivity and sensitivity of this two-particle assay to be useful in a clinical setting. To accomplish this, several parameters were investigated, including nanoparticle size, conjugation chemistry, use of multiple aptamer sequences on the nanoparticles, and use of multiple nanoparticles with different aptamer sequences. After identifying the best-performing elements, the improvements made to this assay’s conditional parameters were combined to illustrate the overall enhanced sensitivity and selectivity of the two-particle assay using an innovative multiple aptamer approach, signifying a critical feature in the advancement of this technique.Keywords
This publication has 19 references indexed in Scilit:
- The PEPvIII-KLH (CDX-110) vaccine in glioblastoma multiforme patientsExpert Opinion on Biological Therapy, 2009
- Prognostic impact of molecular markers in a series of 220 primary glioblastomasCancer, 2006
- Serum protein markers for early detection of ovarian cancerProceedings of the National Academy of Sciences of the United States of America, 2005
- Prognostic Effect of Epidermal Growth Factor Receptor and EGFRvIII in Glioblastoma Multiforme PatientsClinical Cancer Research, 2005
- Nucleic acid aptamers in cancer medicine.FEBS Letters, 2002
- Molecular Aptamer Beacons for Real-Time Protein RecognitionBiochemical and Biophysical Research Communications, 2002
- Human Herpesvirus 6 and Multiple Sclerosis: Systemic Active Infections in Patients with Early DiseaseClinical Infectious Diseases, 2000
- Adaptive Recognition by Nucleic Acid AptamersScience, 2000
- Presentation of prostate tumor antigens by dendritic cells stimulates T-cell proliferation and cytotoxicityThe Prostate, 1996
- Oligonucleotides as Research, Diagnostic, and Therapeutic AgentsOnline Journal of Public Health Informatics, 1995