Comparison of Proliferative and Multilineage Differentiation Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord and Bone Marrow
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- 1 March 2007
- journal article
- research article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 25 (6), 1384-1392
- https://doi.org/10.1634/stemcells.2006-0709
Abstract
Human umbilical cord perivascular cells (HUCPVCs) have been shown to have a high proliferative potential and the capacity to differentiate into an osteogenic phenotype. HUCPVCs have thus been considered a possible extra-embryonic mesenchymal stem cell (MSC) source for cell-based therapies. To assess this potential, we compared HUCPVCs to the “gold standard” bone marrow mesenchymal stromal cells (BMSCs) with respect to their proliferation, differentiation, and transfection capacities. HUCPVCs showed a higher proliferative potential than BMSCs and were capable of osteogenic, chondrogenic, and adipogenic differentiation. Interestingly, osteogenic differentiation of HUCPVCs proceeded more rapidly than BMSCs. Additionally, HUCPVCs expressed higher levels of CD146, a putative MSC marker, relative to BMSCs. HUCPVCs showed comparable transfection efficiency as BMSCs using a nucleofection method but were more amenable to transfection with liposomal methods (FuGENE). Gene array analysis showed that HUCPVCs also expressed Wnt signaling pathway genes that have been implicated in the regulation of MSCs. The similar characteristics between HUCPVCs and MSCs support the applicability of HUCPVCs for cell-based therapies. Disclosure of potential conflicts of interest is found at the end of this article.Keywords
This publication has 25 references indexed in Scilit:
- Regulation of Cartilaginous ECM Gene Transcription by Chondrocytes and MSCs in 3D Culture in Response to Dynamic LoadingBiomechanics and Modeling in Mechanobiology, 2006
- Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic TherapyJournal of Clinical Oncology, 2006
- Hydrogel-β-TCP scaffolds and stem cells for tissue engineering boneBone, 2005
- Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's DiseaseThe International Journal of Cell Cloning, 2005
- Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapyCancer Science, 2005
- Circulating endothelial cellsThrombosis and Haemostasis, 2005
- Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical CordThe International Journal of Cell Cloning, 2004
- Transplantation of porcine umbilical cord matrix cells into the rat brainExperimental Neurology, 2003
- Perivascular Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental PulpJournal of Bone and Mineral Research, 2003
- Bone regeneration by implantation of purified, culture‐expanded human mesenchymal stem cellsJournal of Orthopaedic Research, 1998