Involvement of prostaglandin E2 released from leptomeningeal cells in increased expression of transforming growth factor‐β in glial cells and cortical neurons during systemic inflammation

Abstract

The leptomeninges play a central role in the antiinflammatory response through the glia–neuron interaction during systemic inflammation. In the present study, we examined the possible production of two potent antiinflammatory mediators, prostaglandin E₂ (PGE₂) and transforming growth factor-β1 (TGF-β1) by leptomeningeal cells during systemic inflammation. After immunization with the complete Freund's adjuvant (CFA), cyclooxygenase (COX)-2 and membrane-bound PGE synthase-1 (mPGES-1) were induced in the leptomeninges. Primary cultured leptomeningeal cells secreted PGE₂ after treatment with lipopolysaccharide (LPS) or proinflammatory cytokines. The LPS-induced release of PGE₂ was depressed by a selective COX-2 inhibitor, NS-398. On the other hand, TGF-β1 and TGF-β receptor II (TGF-βRII) both markedly increased in the leptomeninges and the parenchymal cells after the CFA injection. Double-staining immunohistochemistry demonstrated TGF-β1 to be induced in both glial cells and cortical neurons, whereas TGF-βRII was induced only in cortical neurons. Furthermore, the conditioned medium prepared from the leptomeningeal cells after LPS stimulation was able to induce an increased expression of TGF-β1 and TGF-βRII in the primary cultured glial cells and cortical neurons. This increased expression was suppressed by NS-398. PGE₂ was found to increase directly the production of TGF-β1 and TGF-βRII in the primary cultured cells. These observations strongly suggest that PGE₂, which is biosynthesized by the leptomeninges, mainly regulates the production of TGF-β1 by glial cells and cortical neuron, thus playing a protective role in the cortical neurons during systemic inflammation. Furthermore, TGF-β1 may also exert a protective effect directly on the cortical neurons.