Follicular Atresia and Infertility in Rats Treated with a Gonadotropin-Releasing Hormone Antagonist*
- 1 July 1990
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 127 (1), 25-31
- https://doi.org/10.1210/endo-127-1-25
Abstract
Adverse effects of the GnRH antagonist Antide on folliculogenesis and fertility were noted when we were evaluating the therapeutic value of Antide on endometriosis in a rat model. Cyclic rats with (n = 56) and without (n = 18) surgically induced endometriosis received Antide (2 mg/kg) or vehicle at noon on days 0 (proestrus), 3, 6, and 9. Rats were killed at noon on days 0, 6, 12, 18, 24, 30, 42, and 165. The number of antral follicles and the number of atretic antral follicles evaluated did not differ (P > 0.05) between endometriosis and control rats. Antide-treated rats had more (P < 0.05) atretic antral follicles (64.7%) than vehicle-treated rats (15.3%). Antide-treated rat ovaries contained fewer corpora lutea than those of vehicle-treated rats on day 6, 12, and 18. No corpora lutea were found in Antide-treated rat obarious after day 18. The abnormal ovarian morphology of the Antide-treated rats persisted for the duration of the project (165 days). Fertility (rats without endometriosis) was assessed by mating vehicle-and Antide-treated rats at spontaneous proestrus (n = 8) for eight posttreatment cycles as well as after follicular stimulation (n = 2). No oocytes were found in the oviducts of Antide-treated rats after eight cycles, indicating that ovulation had not occurred. The serum FSH and estradiol concentrations in the rats treated with Antide were lower (P < 0.05) on days 6, 12, and 18, but rose to values equal to (days 24 and 30) or greater than (day 42) those in vehicle-treated rats. Serum progesterone levels in rats treated with Antide were lower (P < 0.05) than those in vehicle-treated rats on all days tested. In conclusion, at a dosage sufficient to suppress reproductive cyclicity (and elicit the regression of endometriosis), Antide also caused long term follicular atresia and infertility.This publication has 8 references indexed in Scilit:
- GnRH RECEPTORS IN HUMAN GRANULOSA CELLS: ANATOMICAL LOCALIZATION AND CHARACTERIZATION BY AUTORADIOGRAPHIC STUDYEndocrinology, 1989
- Effects of long-term administration of a superactive agonistic and an antagonistic GnRH analog on the pituitary-gonad systemPeptides, 1989
- PROLONGED DURATION OF GONADOTROPIN INHIBITION BY A THIRD GENERATION GNRH ANTAGONIST*&Journal of Clinical Endocrinology & Metabolism, 1988
- Design, synthesis and bioassays of antagonists of LHRH which have high antiovulatory activity and release negligible histamineBiochemical and Biophysical Research Communications, 1987
- Studies on the surgical induction of endometriosis in the ratFertility and Sterility, 1985
- Specific binding of luteinizing hormone releasing hormone to human luteal tissueBiochemical and Biophysical Research Communications, 1983
- INHIBITION OF HUMAN GRANULOSA CELL PROGESTERONE SECRETION BY A GONADOTROPIN-RELEASING HORMONE AGONISTJournal of Clinical Endocrinology & Metabolism, 1982
- The effect of luteinizing hormone-releasing factor and its agonist on cultured human granulosa cellsFertility and Sterility, 1982