Transcription from a TATA-less promoter requires a multisubunit TFIID complex.
Open Access
- 1 November 1991
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 5 (11), 1935-1945
- https://doi.org/10.1101/gad.5.11.1935
Abstract
In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).Keywords
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