Cloning of a Transcriptionally Active Human TATA Binding Factor
- 29 June 1990
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 248 (4963), 1646-1650
- https://doi.org/10.1126/science.2194289
Abstract
Transcription factor IID (TFIID) binds to the TATA box promoter element and regulates the expression of most eukaryotic genes transcribed by RNA polymerase II. Complementary DNA (cDNA) encoding a human TFIID protein has been cloned. The human TFIID polypeptide has 339 amino acids and a molecular size of 37,745 daltons. The carboxyl-terminal 181 amino acids of the human TFIID protein shares 80% identity with the TFIID protein from Saccharomyces cerevisiae. The amino terminus contains an unusual repeat of 38 consecutive glutamine residues and an X-Thr-Pro repeat. Expression of DNA in reticulocyte lysates or in Escherichia coli yielded a protein that was competent for both DNA binding and transcription activation.Keywords
This publication has 23 references indexed in Scilit:
- SPT15, the gene encoding the yeast TATA binding factor TFIID, is required for normal transcription initiation in vivoCell, 1989
- Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertionsCell, 1989
- Cloning and structure of a yeast gene encoding a general transcription initiation factor TFIID that binds to the TATA boxNature, 1989
- Physical Analysis of Transcription Preinitiation Complex Assembly on a Class II Gene PromoterScience, 1988
- Function of a yeast TATA element-binding protein in a mammalian transcription systemNature, 1988
- A yeast activity can substitute for the HeLa cell TATA box factorNature, 1988
- A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoterNature, 1987
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesJournal of Molecular Biology, 1986
- Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequencesNature, 1983
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977