Photometric microplate assay for estimation of the efficacy of paraoxon-inhibited acetylcholinesterase reactivation
- 1 January 2008
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Enzyme Inhibition and Medicinal Chemistry
- Vol. 23 (6), 781-784
- https://doi.org/10.1080/14756360701811023
Abstract
Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE) using three reactivators for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained using obidoxime where reactivation was near to 80%. Suitability of photometric microplates for following of reactivation procedures is discussed.Keywords
This publication has 17 references indexed in Scilit:
- Amperometric Biosensor for Evaluation of Competitive Cholinesterase Inhibition by the Reactivator HI‐6Analytical Letters, 2007
- Currently Used Cholinesterase Reactivators Against Nerve Agent Intoxication: Comparison of Their Effectivity in VitroDrug and Chemical Toxicology, 2007
- A comparison of reactivating efficacy of newly developed oximes (K074, K075) and currently available oximes (obidoxime, HI-6) in cyclosarin-and tabun-poisoned ratsJournal of Enzyme Inhibition and Medicinal Chemistry, 2007
- Mineralization of Paraoxon and Its Use as a Sole C and P Source by a Rationally Designed Catabolic Pathway in Pseudomonas putidaApplied and Environmental Microbiology, 2006
- Tests withDaphnia magna: A new approach to prescreen toxicity of newly synthesized acetylcholinesterase reactivatorsJournal of Enzyme Inhibition and Medicinal Chemistry, 2006
- New group of xylene linker-containing acetylcholinesterase reactivators as antidotes against the nerve agent cyclosarinJournal of Enzyme Inhibition and Medicinal Chemistry, 2006
- Potency of new structurally different oximes to reactivate cyclosarin-inhibited human brain acetylcholinesterasesJournal of Enzyme Inhibition and Medicinal Chemistry, 2006
- In vitroreactivation of sarin-inhibited brain acetylcholinesterase from different species by various oximesJournal of Enzyme Inhibition and Medicinal Chemistry, 2005
- A new group of monoquaternary reactivators of acetylcholinesterase inhibited by nerve agentsJournal of Enzyme Inhibition and Medicinal Chemistry, 2005
- Reactivation of Cyclosarin-inhibited Rat Brain Acetylcholinesterase by Pyridinium–OximesJournal of Enzyme Inhibition and Medicinal Chemistry, 2004