Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco.
- 1 October 1986
- journal article
- Vol. 5 (10), 2419-25
Abstract
A gene encoding the small subunit of ribulose 1,5-bisphosphate carboxylase (rbcS) in wheat, a monocot plant, was transferred to tobacco, a dicot plant. The wheat gene is not expressed in transgenic tobacco under the control of its own promoter, but when transcription is driven by a viral promoter, several wheat transcripts accumulate. These include both spliced and unspliced transcripts, which are polyadenylated at multiple novel sites in the wheat 3' flanking region. Another monocot intron, from the maize Adh-1 gene, is also spliced inefficiently in tobacco. These findings contrast results demonstrating efficient processing of different rbcS transcripts from pea, a dicot, in transgenic tobacco. This pattern may reflect general differences in sequences required for RNA processing in monocot and dicot plants.Keywords
This publication has 46 references indexed in Scilit:
- The "Spliceosome": Yeast Pre-Messenger RNA Associates with a 40 S Complex in a Splicing-Dependent ReactionScience, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Lariat structures are in vivo intermediates in yeast pre-mRNA splicingCell, 1984
- Requirement of a downstream sequence for generation of a poly(A) addition siteCell, 1984
- Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extractsCell, 1983
- Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progenyCell, 1983
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974
- Isolation of mRNA from KB‐Cells by Affinity Chromatography on Polyuridylic Acid Covalently Linked to SepharoseEuropean Journal of Biochemistry, 1972