DNA methylation analysis on satellite I region in blastocysts obtained from somatic cell cloned cattle
- 20 April 2011
- journal article
- Published by Wiley in Animal Science Journal
- Vol. 82 (4), 523-530
- https://doi.org/10.1111/j.1740-0929.2011.00881.x
Abstract
Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non-cloned or cloned dams using semen from non-cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non-cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non-cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle.Keywords
This publication has 33 references indexed in Scilit:
- Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competenceMolecular Reproduction and Development, 2010
- Death losses due to stillbirth, neonatal death and diseases in cloned cattle derived from somatic cell nuclear transfer and their progeny: a result of nationwide survey in JapanAnimal Science Journal, 2009
- In vitroculture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre- and post-implantation stages of development in cattleBMC Developmental Biology, 2009
- Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a DecadePLOS ONE, 2009
- QUMA: quantification tool for methylation analysisNucleic Acids Research, 2008
- Belated approval of first recombinant protein from animalNature Biotechnology, 2006
- Inheritable Histone H4 Acetylation of Somatic Chromatins in Cloned EmbryosOnline Journal of Public Health Informatics, 2006
- Variation in Gene Expression and Aberrantly Regulated Chromosome Regions in Cloned Mice1Biology of Reproduction, 2005
- Varied patterns of DNA methylation change between different satellite regions in bovine preimplantation developmentMolecular Reproduction and Development, 2005
- A Preadipocyte Clonal Line from Bovine Intramuscular Adipose Tissue: Nonexpression of GLUT-4 Protein during Adipocyte DifferentiationBiochemical and Biophysical Research Communications, 1995