In vitroculture and somatic cell nuclear transfer affect imprinting of SNRPN gene in pre- and post-implantation stages of development in cattle
Open Access
- 6 February 2009
- journal article
- Published by Springer Science and Business Media LLC in BMC Developmental Biology
- Vol. 9 (1), 9
- https://doi.org/10.1186/1471-213x-9-9
Abstract
Embryo in vitro manipulations during early development are thought to increase mortality by altering the epigenetic regulation of some imprinted genes. Using a bovine interspecies model with a single nucleotide polymorphism, we assessed the imprinting status of the small nuclear ribonucleoprotein polypeptide N (SNRPN) gene in bovine embryos produced by artificial insemination (AI), in vitro culture (IVF) and somatic cell nuclear transfer (SCNT) and correlated allelic expression with the DNA methylation patterns of a differentially methylated region (DMR) located on the SNRPN promoter.This publication has 29 references indexed in Scilit:
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